Presentation Title
Clustering of Epigenetic Methylation and Oxidative Damage: effects on duplex DNA thermodynamic stability, solution structure and local dynamics
Presentation Type
Oral Presentation
Abstract
Deoxyribonucleic acid (DNA) is a macromolecule composed of two biopolymer strands responsible for storing genetic information. Like many other biological molecules, its structure and dynamics are intimately related to its function. Cytosine methylation results in the formation of 5-methylcytosine (5mC), a principal epigenetic regulatory modification. In parallel, DNA oxidation commonly results in 8-oxoguanine (oxoG) a mutagenic adduct. This presentation reports on changes in NMR solution structure and local dynamics due to clustering of 5mC and oxoG in a CpG site. In addition, principal component analysis was used on molecular dynamics trajectories to determine changes in the dominant modes of motion.
Start Date
6-5-2017 2:00 PM
End Date
6-5-2017 2:15 PM
Genre/Form
presentations (communicative events)
Subjects - Topical (LCSH)
DNA--Methylation; DNA damage; DNA repair; Active oxygen in the body
Type
Event
Format
application/pdf
Language
English
Clustering of Epigenetic Methylation and Oxidative Damage: effects on duplex DNA thermodynamic stability, solution structure and local dynamics
Miller Hall
Deoxyribonucleic acid (DNA) is a macromolecule composed of two biopolymer strands responsible for storing genetic information. Like many other biological molecules, its structure and dynamics are intimately related to its function. Cytosine methylation results in the formation of 5-methylcytosine (5mC), a principal epigenetic regulatory modification. In parallel, DNA oxidation commonly results in 8-oxoguanine (oxoG) a mutagenic adduct. This presentation reports on changes in NMR solution structure and local dynamics due to clustering of 5mC and oxoG in a CpG site. In addition, principal component analysis was used on molecular dynamics trajectories to determine changes in the dominant modes of motion.