Presentation Title

Clustering of Epigenetic Methylation and Oxidative Damage: effects on duplex DNA thermodynamic stability, solution structure and local dynamics

Presentation Type

Oral Presentation

Abstract

Deoxyribonucleic acid (DNA) is a macromolecule composed of two biopolymer strands responsible for storing genetic information. Like many other biological molecules, its structure and dynamics are intimately related to its function. Cytosine methylation results in the formation of 5-methylcytosine (5mC), a principal epigenetic regulatory modification. In parallel, DNA oxidation commonly results in 8-oxoguanine (oxoG) a mutagenic adduct. This presentation reports on changes in NMR solution structure and local dynamics due to clustering of 5mC and oxoG in a CpG site. In addition, principal component analysis was used on molecular dynamics trajectories to determine changes in the dominant modes of motion.

Start Date

6-5-2017 2:00 PM

End Date

6-5-2017 2:15 PM

Location

Miller Hall

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May 6th, 2:00 PM May 6th, 2:15 PM

Clustering of Epigenetic Methylation and Oxidative Damage: effects on duplex DNA thermodynamic stability, solution structure and local dynamics

Miller Hall

Deoxyribonucleic acid (DNA) is a macromolecule composed of two biopolymer strands responsible for storing genetic information. Like many other biological molecules, its structure and dynamics are intimately related to its function. Cytosine methylation results in the formation of 5-methylcytosine (5mC), a principal epigenetic regulatory modification. In parallel, DNA oxidation commonly results in 8-oxoguanine (oxoG) a mutagenic adduct. This presentation reports on changes in NMR solution structure and local dynamics due to clustering of 5mC and oxoG in a CpG site. In addition, principal component analysis was used on molecular dynamics trajectories to determine changes in the dominant modes of motion.