Document Type

Article

Publication Date

1-2015

Keywords

Cryphonectria parasitica, Hypovirulence, Hypovirus

Abstract

The impact of chestnut blight, caused by the fungal pathogen Cryphonectria parasitica, has diminished in Europe due to a natural biological control caused by hypovirus infection. Hypovirulence- mediated biological control has been far less successful in North America meriting further evaluation of field isolates that have the ability to produce non-lethal cankers, generate hypovirulent inoculum, and exhibit a greater ecological fitness in forest systems. In this study, Cryphonectria hypoviruses (CHV) CHV3-County Line, CHV1-Euro7, and CHV1-Ep713 were evaluated in five different isolates of C. parasitica. One hundred and eighty cankers representing each treatment combination were initiated on American chestnut sprouts in the Monongahela National Forest, West Virginia, USA. The size of cankers, the persistence of hypovirulent (HV) isolates, stroma production, and hypovirus transmission to conidia were assessed four and 12 months after canker expansion. CHV3-County Line infected isolates produced significantly smaller cankers than the isolates infected with either CHV1-type. With regard to CHV1-Euro7 isolates, the fungal genome appeared to contribute to the differences in canker size. After four months, HV isolates harboring either CHV1-type (30%) were retrieved at a significantly higher rate than isolates containing CHV3-County Line (14%). After 12 months, the HV recovery was similar among the three hypoviruses indicating smaller cankers will maintain their HV status after one year. Very few stroma were produced after one year in the field from HV isolates. In vitro, CHV3-County Line (49%) had a significantly lower rate of hypovirus transmission to conidia when compared to CHV1-Euro7 (87%) and CHV1-Ep713 (80%). Significant differences existed among the five different isolates indicating HV transmission is dependent on the fungal genome. This research provided additional evidence that each hypovirus interacts with its host differently and certain isolate/hypovirus combinations have better biological control potential than others.

Publication Title

American Journal of Plant Sciences

Volume

6

First Page

73

Last Page

83

Required Publisher's Statement

Published Online in Scientific Research Publishing

http://dx.doi.org/10.4236/ajps.2015.61009

Subjects - Topical (LCSH)

Endothia parasitica--Control--West Virginia--Monongahela National Forest; Chestnut blight--Biological control--West Virginia--Monongahela National Forest; American chestnut--Diseases and pests--Biological control--West Virginia--Monongahela National Forest; Chestnut blight--West Virginia--Monongahela National Forest

Geographic Coverage

Monongahela National Forest (W. Va.)

Genre/Form

articles

Type

Text

Creative Commons License

Creative Commons Attribution 4.0 International License
This work is licensed under a Creative Commons Attribution 4.0 International License.

Language

English

Format

application/pdf

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