Document Type

Article

Publication Date

9-2012

Keywords

aflatoxin; oxylipin; G protein-coupled receptor (GPCR); sclerotia

Abstract

Oxylipins regulate Aspergillus development and mycotoxin production and are also involved inAspergillus quorum sensing mechanisms. Despite extensive knowledge of how these oxylipins are synthesized and what processes they regulate, nothing is known about how these signals are detected and transmitted by the fungus. G protein-coupled receptors (GPCR) have been speculated to be involved as they are known oxylipin receptors in mammals, and many putative GPCRs have been identified in the Aspergilli. Here, we present evidence that oxylipins stimulate a burst in cAMP in A. nidulans, and that loss of an A. nidulans GPCR, gprD, prevents this cAMP accumulation. A. flavus undergoes an oxylipin-mediated developmental shift when grown at different densities, and this regulates spore, sclerotial and aflatoxin production. A. flavus encodes two putative GprD homologs, GprC and GprD, and we demonstrate here that they are required to transition to a high-density development state, as well as to respond to spent medium of a high-density culture. The finding of GPCRs that regulate production of survival structures (sclerotia), inoculum (spores) and aflatoxin holds promise for future development of anti-fungal therapeutics.

Publication Title

Toxins

Volume

4

Issue

9

First Page

695

Last Page

717

Comments

© 2012 MDPI

Subjects - Topical (LCSH)

Aspergillus--Microbiology; Aspergillus; Quorum sensing (Microbology)

Genre/Form

articles

Type

Text

Creative Commons License

Creative Commons Attribution 3.0 License
This work is licensed under a Creative Commons Attribution 3.0 License.

Language

English

Format

application/pdf

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