Finding genes with cis-acting mutations in S. cerevisiae
Research Mentor(s)
Dan Pollard
Description
This study seeks to provide a foundation for studying genes with mutations that regulate the expression of the genes they are found in. This study was conducted with two strains of the model organism Saccharomyces cerevisiae (yeast). The “lab” strain was isolated from a fig and the “clinical” strain was originally isolated from a patient’s lung. Abundance of gene expression was collected at three time points after pheremone response that serves as a model for how animal and plant cells respond to changing environmental conditions. Gene expression was measured through RNA abundance and Protein abundance at each timepoint. Statistical analysis and clustering were used to identify genes of interest. The characteristics for our genes of interest are non-significant changes in RNA abundance over time, and significant changes in protein abundance over time. The information found in this study will be used to select genes for further study and find the location of the mutations responsible for the gene’s regulation.
Document Type
Event
Start Date
May 2022
End Date
May 2022
Location
Carver Gym (Bellingham, Wash.)
Department
CSE - Biology
Genre/Form
student projects; posters
Type
Image
Rights
Copying of this document in whole or in part is allowable only for scholarly purposes. It is understood, however, that any copying or publication of this document for commercial purposes, or for financial gain, shall not be allowed without the author’s written permission.
Language
English
Format
application/pdf
Finding genes with cis-acting mutations in S. cerevisiae
Carver Gym (Bellingham, Wash.)
This study seeks to provide a foundation for studying genes with mutations that regulate the expression of the genes they are found in. This study was conducted with two strains of the model organism Saccharomyces cerevisiae (yeast). The “lab” strain was isolated from a fig and the “clinical” strain was originally isolated from a patient’s lung. Abundance of gene expression was collected at three time points after pheremone response that serves as a model for how animal and plant cells respond to changing environmental conditions. Gene expression was measured through RNA abundance and Protein abundance at each timepoint. Statistical analysis and clustering were used to identify genes of interest. The characteristics for our genes of interest are non-significant changes in RNA abundance over time, and significant changes in protein abundance over time. The information found in this study will be used to select genes for further study and find the location of the mutations responsible for the gene’s regulation.