Event Title

Monitoring Diarrhetic Shellfish Poisoning (DSP) in Washington

Presentation Abstract

Monitoring for diarrhetic shellfish toxins (DSTs) was initiated in the summer of 2011 when the first illness due to diarrhetic shellfish poisoning (DSP) was reported in the state of Washington. Monitoring strategy includes collection of whole seawater for identification and enumeration of Dinophysis and several shellfish species collected from two sites in Sequim Bay, WA as well as other sites throughout Puget Sound during the following summer (2012). Shellfish samples were analyzed for Okadaic acid toxin group including DTX-1, DTX-2 and DTX-3 using rapid screening methods based on the functional assays, i.e. the protein phosphatase 2A inhibition assay (PP2A); the anti OA antibody in an enzyme-linked immunosorbent assay (ELISA) and a lateral flow test strip (Jellett Rapid Test) and the chemical method, i.e. liquid chromatography coupled with tandem mass spectroscopy (LC-MS/MS). The application of a rapid screening method along with Dinophysis cell counts can provide quick assessment of toxin levels, and the results of this study suggest that PP2A is better at screening for OA group of DSTs. The reason for this is due to the fact that DSTs in Washington consist mostly as DTX-1; test kits with antibody-based application tend to under-estimate toxin levels present because the antibody used in these tests is specific to Okadaic acid (100% cross-reactivity) and only 50% to DTX-1.

Session Title

Session S-08A: Harmful Algal Blooms, Climate, Shellfish, and Public Health - Emerging Issues in a Changing World

Conference Track

Harmful Algal Blooms and Shellfish

Conference Name

Salish Sea Ecosystem Conference (2014 : Seattle, Wash.)

Document Type

Event

Start Date

1-5-2014 5:00 PM

End Date

1-5-2014 6:30 PM

Location

Room 6C

Contributing Repository

Digital content made available by University Archives, Heritage Resources, Western Libraries, Western Washington University.

Rights

This resource is displayed for educational purposes only and may be subject to U.S. and international copyright laws. For more information about rights or obtaining copies of this resource, please contact University Archives, Heritage Resources, Western Libraries, Western Washington University, Bellingham, WA 98225-9103, USA (360-650-7534; heritage.resources@wwu.edu) and refer to the collection name and identifier. Any materials cited must be attributed to the Salish Sea Ecosystem Conference Records, University Archives, Heritage Resources, Western Libraries, Western Washington University.

Type

Text

Language

English

Format

application/pdf

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May 1st, 5:00 PM May 1st, 6:30 PM

Monitoring Diarrhetic Shellfish Poisoning (DSP) in Washington

Room 6C

Monitoring for diarrhetic shellfish toxins (DSTs) was initiated in the summer of 2011 when the first illness due to diarrhetic shellfish poisoning (DSP) was reported in the state of Washington. Monitoring strategy includes collection of whole seawater for identification and enumeration of Dinophysis and several shellfish species collected from two sites in Sequim Bay, WA as well as other sites throughout Puget Sound during the following summer (2012). Shellfish samples were analyzed for Okadaic acid toxin group including DTX-1, DTX-2 and DTX-3 using rapid screening methods based on the functional assays, i.e. the protein phosphatase 2A inhibition assay (PP2A); the anti OA antibody in an enzyme-linked immunosorbent assay (ELISA) and a lateral flow test strip (Jellett Rapid Test) and the chemical method, i.e. liquid chromatography coupled with tandem mass spectroscopy (LC-MS/MS). The application of a rapid screening method along with Dinophysis cell counts can provide quick assessment of toxin levels, and the results of this study suggest that PP2A is better at screening for OA group of DSTs. The reason for this is due to the fact that DSTs in Washington consist mostly as DTX-1; test kits with antibody-based application tend to under-estimate toxin levels present because the antibody used in these tests is specific to Okadaic acid (100% cross-reactivity) and only 50% to DTX-1.