Event Title

Bacterial Diversity at Anthropogenic Sulfide Seeps in Commencement Bay, WA

Presentation Abstract

We have observed filamentous bacteria on the surface of sediments and other substrates (e.g., rocks, crabs) in nearshore areas of Commencement Bay, WA. These areas have been found to have high amounts of wood debris (from former sawmills) buried in the sediments, and decomposition by sulfate-reducing bacteria has created a very high sulfide environment (> 1 mM) in sediment porewater and where streams of water are seeping from intertidal sediments at low tide. The objective of this study was to use microscopy (SEM, FISH) and genetic methods (cloning and sequencing, next generation sequencing) to compare the diversity, distribution, and abundance of bacteria on these different substrates. There were significant differences in the types of bacteria and their relative abundances between sand, rocks and crabs. Sulfide-oxidizing bacteria were the most common members of the microbial communities, and they were closely related to those found on deep sea hydrothermal vents. These microbial communities are amenable to detailed experimental study, which will provide insight into ecological relationships of microbes in high sulfide environments.

Session Title

General species and food webs

Conference Track

Species and Food Webs

Conference Name

Salish Sea Ecosystem Conference (2016 : Vancouver, B.C.)

Document Type

Event

Location

2016SSEC

Type of Presentation

Poster

Contributing Repository

Digital content made available by University Archives, Heritage Resources, Western Libraries, Western Washington University.

Rights

This resource is displayed for educational purposes only and may be subject to U.S. and international copyright laws. For more information about rights or obtaining copies of this resource, please contact University Archives, Heritage Resources, Western Libraries, Western Washington University, Bellingham, WA 98225-9103, USA (360-650-7534; heritage.resources@wwu.edu) and refer to the collection name and identifier. Any materials cited must be attributed to the Salish Sea Ecosystem Conference Records, University Archives, Heritage Resources, Western Libraries, Western Washington University.

Type

Text

Language

English

Format

application/pdf

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Bacterial Diversity at Anthropogenic Sulfide Seeps in Commencement Bay, WA

2016SSEC

We have observed filamentous bacteria on the surface of sediments and other substrates (e.g., rocks, crabs) in nearshore areas of Commencement Bay, WA. These areas have been found to have high amounts of wood debris (from former sawmills) buried in the sediments, and decomposition by sulfate-reducing bacteria has created a very high sulfide environment (> 1 mM) in sediment porewater and where streams of water are seeping from intertidal sediments at low tide. The objective of this study was to use microscopy (SEM, FISH) and genetic methods (cloning and sequencing, next generation sequencing) to compare the diversity, distribution, and abundance of bacteria on these different substrates. There were significant differences in the types of bacteria and their relative abundances between sand, rocks and crabs. Sulfide-oxidizing bacteria were the most common members of the microbial communities, and they were closely related to those found on deep sea hydrothermal vents. These microbial communities are amenable to detailed experimental study, which will provide insight into ecological relationships of microbes in high sulfide environments.