Presentation Abstract

Taxonomic analysis using the compound microscope remains an efficient and commonly used method of monitoring phytoplankton in seawater samples. Certain diatoms in the Pseudo-nitzschia genus produce the toxin domoic acid, and thereby cause amnesic shellfish poisoning or domoic acid poisoning. Because differentiation between Pseudo-nitzschia species using the compound microscope is challenging, often routine sample analysis in the past has only noted these algae to genus level. This makes it difficult to assess if there is a range shift in the more toxic species, such as Pseudo-nitzschia australis, that may be linked to alterations in environmental conditions in the Salish Sea due to climate change. We present here a practical method for identifying P. australis in the light microscope, and suggest groupings that can be commonly used to monitor other local Pseudo-nitzschia species.

Session Title

Harmful Phytoplankton in the Salish Sea: Part II

Keywords

HABs, Pseudo-nitzschia, Microscopic identification

Conference Track

SSE5: Climate Change: Impacts, Adaptation, and Research

Conference Name

Salish Sea Ecosystem Conference (Seattle, WA : 2018)

Document Type

Event

SSEC Identifier

SSE5-140

Start Date

4-4-2018 4:15 PM

End Date

4-4-2018 4:30 PM

Type of Presentation

Oral

Contributing Repository

Digital content made available by University Archives, Heritage Resources, Western Libraries, Western Washington University.

Geographic Coverage

Salish Sea (B.C. and Wash.)

Rights

This resource is displayed for educational purposes only and may be subject to U.S. and international copyright laws. For more information about rights or obtaining copies of this resource, please contact University Archives, Heritage Resources, Western Libraries, Western Washington University, Bellingham, WA 98225-9103, USA (360-650-7534; heritage.resources@wwu.edu) and refer to the collection name and identifier. Any materials cited must be attributed to the Salish Sea Ecosystem Conference Records, University Archives, Heritage Resources, Western Libraries, Western Washington University.

Type

text

Language

English

Format

application/pdf

Share

COinS
 
Apr 4th, 4:15 PM Apr 4th, 4:30 PM

Differentiation of pseudo-nitzschia species (Baccillariophyceae) in seawater samples from the Salish Sea using the compound microscope

Taxonomic analysis using the compound microscope remains an efficient and commonly used method of monitoring phytoplankton in seawater samples. Certain diatoms in the Pseudo-nitzschia genus produce the toxin domoic acid, and thereby cause amnesic shellfish poisoning or domoic acid poisoning. Because differentiation between Pseudo-nitzschia species using the compound microscope is challenging, often routine sample analysis in the past has only noted these algae to genus level. This makes it difficult to assess if there is a range shift in the more toxic species, such as Pseudo-nitzschia australis, that may be linked to alterations in environmental conditions in the Salish Sea due to climate change. We present here a practical method for identifying P. australis in the light microscope, and suggest groupings that can be commonly used to monitor other local Pseudo-nitzschia species.