Senior Project Advisor
The satellite RNA found with tobacco ringspot virus is capable of attenuating the symptoms of viral infection. Although the satellite appears within viral capsids after several passages of infected plants, the origin of this satellite RNA sequence has not been previously determined. Earlier work indicated a potential mitochondrial origin of this sequence. Using satellite specific primers and the polymerase chain reaction (PCR), the mitochondrial DNA of uninfected tobacco cells was probed for sequence corresponding to the satellite RNA. A new protocol was adapted for the isolation of mitochondrial DNA in which specific isolation of protoplasts and mitochondria was not required. Although two PCR products were isolated, sequencing of these products and analysis with the MacVector 3.5 Sequence Analysis program showed no homology with the budblight strain of tobacco ringspot virus satellite RNA.
Gilberd, Jennifer J. (Jennifer Jeane), "Satellite RNA of Tobacco Ringspot Virus" (2000). WWU Honors College Senior Projects. 226.
Subjects - Topical (LCSH)
Tobacco ringspot virus; RNA viruses--Reproduction; Mitochondrial DNA
student projects; term papers
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