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Date Permissions Signed
Date of Award
Master of Science (MS)
Bodensteiner, Leo R.,1957-
Sofield, Ruth M.
Biological structures that develop incremental growth patterns over time present a unique opportunity to study chronological aspects of the organism's chemical environment. Spiny Dogfish (Squalus suckleyi), an abundant shark species, develop two dorsal spines that exhibit this type of growth pattern. The growth patterns on these spines have been used extensively as indicators of age. However, the chronological patterns of trace metal deposits in these spines have yet to be assessed. The main goals of this study were to develop the methods for analyzing this chronology and to explore techniques to analyze these data. Laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) is a recently developed analytical technique for studying spatially-distributed elemental compositions in solids. LA-ICP-MS was used to quantify the concentrations of zinc and strontium across the life histories of 18 Spiny Dogfish. Metal accumulation and size differed between sharks caught at two sampling locations. This method was able to chronologically relate metal deposition to age in individuals of this species.
Western Washington University
Subject – LCSH
Spiny dogfish--Effect of water pollution on; Spiny dogfish--Effect of heavy metals on; Heavy metals--Bioaccumulation; Laser ablation; Inductively coupled plasma mass spectrometry
Copying of this document in whole or in part is allowable only for scholarly purposes. It is understood, however, that any copying or publication of this thesis for commercial purposes, or for financial gain, shall not be allowed without the author's written permission.
Bailes, Clayton L. (Clayton Louis), "Non-lethal determination of heavy metals in spiny dogfish (Squalus suckleyi) spines using LA-ICP-MS" (2012). WWU Graduate School Collection. 238.