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Date Permissions Signed
Date of Award
Department or Program Affiliation
Master of Science (MS)
Moyer, Craig L.
Young, Jeff C. (Jeffery C.)
The primary production by Zetaproteobacteria at iron-rich vents of the Mariana Arc and back-arc supports high microbial diversity. In microaerophilic circumneutral pH, Zetaproteobacteria are able to produce energy by oxidizing ferrous iron (Fe2+) from the vent effluent with the presence of a key gene involved in their iron-oxidation, Cyc2, a fused cytochrome-porin. These genes are found in all Zetaproteobacteria known to date. This study combines molecular quantitative techniques with metagenomic analyses to enhance our confidence in gene quantification from environmental samples. The occurrence of cyc2 was quantified from microbial mat communities at NW Eifuku, NW Rota-1, Snail and Urashima Vents using primers designed from a composite metagenome to better understand the potential impact of Zetaproteobacteria metabolism on these iron-rich microbial communities. Based on a multiple sequence alignment and phylogenetic analysis, two distinct groups of the Zetaproteobacteria cyc2 were identified and termed Cluster 1 cyc2 and Cluster 3 cyc2 based on the nomenclature provided by Chan et al. (2018). Multidimensional scaling of Zetaproteobacteria SSU rRNA and cyc2 gene abundances showed that the presence of the two cyc2 sequence variants are strongly driven by an array of different vent fluid chemicals. We also report on the phylogenetic relationship between the two cyc2 variants and other representative FeOB, as well as the distribution of cyc2 gene abundances across the Mariana hydrothermal vent sites along the Arc and back-arc. Overall, this provides insight on the ecological and evolutionary implications of Zetaproteobacteria iron-oxidation represented at the community-level across a wide-range of geochemical parameters.
Functional genes, Microbial communities, Quantitative polymerase chain reaction
Western Washington University
Subject – LCSH
Proteobacteria--Philippine Sea--Genetics; Metagenomics--Philippine Sea; Hydrothermal vents--Microbiology--Philippine Sea
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Turner, Christina A., "Functional Analysis of Cyc2 in Microbial Mat Communities of Mariana Arc and Back-arc Hydrothermal Vents" (2019). WWU Graduate School Collection. 906.