Title
Applications of Pooled DNA Samples to the Assessment of Population Affinities: Short Tandem Repeats
Document Type
Article
Publication Date
12-2005
Keywords
Pooled DNA, Short tandem repeats, Genetic diversity, R matrix analysis, Chuvash, Buryats, Kizhi, Native Americans, South Africans, New York City whites
Abstract
Pooled DNA samples have been used in association studies of Mendelian disease genes. This method involves combining equal quantities of DNA from patients and control subjects into separate pools and comparing the pools for distributions of genetic markers. In this study identical quantities of DNA from 300 individuals representing 6 populations were pooled and amplified for 296 loci using the touchdown polymerase chain reaction (PCR) method. The purpose of this study is to test the efficacy of pooled DNA markers in the reconstruction of the genetic structure of human populations. The populations sampled included Chuvash, Buryats, Kizhi, Native Americans, South Africans, and New York City whites. To test the accuracy of the allele-frequency distributions, we genotyped the Buryats and New York samples individually for six microsatellite markers and compared their frequen- cies to the allele frequencies derived from the electropherogram peak heights for the pooled DNA, producing a correlation of 0.9811 with a variance of less than 0.04. Two-dimensional scaling of genetic distances among the six populations produced clusters that reflected known historical relationships. A distance matrix was created using all 296 loci, and matrices based on individual chromosomes were correlated against the total matrix. As expected, the largest chromosomes had the highest correlations with the total matrix, whereas one of the smallest chromosomes, chromosome 22, had the lowest correlation and differed most from the combined STR distance matrix.
Publication Title
Human Biology
Volume
77
Issue
6
First Page
723
Last Page
733
Required Publisher's Statement
Published by: Wayne State University Press
Stable URL: https://www.jstor.org/stable/41466370
Recommended Citation
Crawford, M. H.; Banerjee, P.; Demarchi, D. A.; Zlojutro, M.; McComb, J.; Livshits, G.; Henneberg, M.; Mosher, M.J.; Schanfield, M. S.; and Knowles, J. A., "Applications of Pooled DNA Samples to the Assessment of Population Affinities: Short Tandem Repeats" (2005). Anthropology Faculty and Staff Publications. 19.
https://cedar.wwu.edu/anthropology_facpubs/19
Subjects - Topical (LCSH)
Germplasm resources--Utilization; DNA--Analysis; Genetic markers
Genre/Form
articles
Type
Text
Rights
Copying of this document in whole or in part is allowable only for scholarly purposes. It is understood, however, that any copying or publication of this document for commercial purposes, or for financial gain, shall not be allowed without the author’s written permission.
Language
English
Format
application/pdf
