Document Type
Article
Publication Date
5-2016
Keywords
Chloroflexi, Single-cell genomics
Abstract
Chloroflexi small-subunit (SSU) rRNA gene sequences are frequently recovered from subseafloor environments, but the metabolic potential of the phylum is poorly understood. The phylum Chloroflexi is represented by isolates with diverse metabolic strategies, including anoxic phototrophy, fermentation, and reductive dehalogenation; therefore, function cannot be attributed to these organisms based solely on phylogeny. Single-cell genomics can provide metabolic insights into uncultured organisms, like the deep-subsurface Chloroflexi. Nine SSU rRNA gene sequences were identified from single-cell sorts of whole-round core material collected from the Okinawa Trough at Iheya North hydrothermal field as part of Integrated Ocean Drilling Program (IODP) expedition 331 (Deep Hot Biosphere). Previous studies of subsurface Chloroflexi single amplified genomes (SAGs) suggested heterotrophic or lithotrophic metabolisms and provided no evidence for growth by reductive dehalogenation. Our nine Chloroflexi SAGs (seven of which are from the order Anaerolineales) indicate that, in addition to genes for the Wood-Ljungdahl pathway, exogenous carbon sources can be actively transported into cells. At least one subunit for pyruvate ferredoxin oxidoreductase was found in four of the Chloroflexi SAGs. This protein can provide a link between the Wood-Ljungdahl pathway and other carbon anabolic pathways. Finally, one of the seven Anaerolineales SAGs contains a distinct reductive dehalogenase homologous (rdhA) gene.
Publication Title
Applied and Environmental Microbiology
Volume
82
Issue
10
First Page
3000
Last Page
3008
Required Publisher's Statement
Published by the American Society for Microbiology
Link to publishers version: http://aem.asm.org/content/82/10/3000.full
Copyright © 2016 Fullerton and Moyer. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.
doi:10.1128/AEM.00624-16
Recommended Citation
Moyer, Craig L., "Comparative Single-Cell Genomics of Chloroflexi from the Okinawa Trough Deep-Subsurface Biosphere" (2016). Biology Faculty and Staff Publications. 50.
https://cedar.wwu.edu/biology_facpubs/50
Subjects - Topical (LCSH)
Back-arc basins--East China Sea; Microbial genomics; Hydrothermal vents; Deep-sea ecology
Subjects - Names (LCNAF)
Integrated Ocean Drilling Program
Geographic Coverage
East China Sea
Supplemental material
Genre/Form
articles
Type
Text
Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.
Language
English
Format
application/pdf
