Physiological and ecological implications of an iron- or hydrogen-oxidizing member of the Zetaproteobacteria, Ghiorsea bivora, gen. nov., sp. nov.

Authors

Jiro F. Mori, Friedrich Schiller University, Jena
Jarrod J. Scott, Bigelow Laboratory for Ocean Sciences
Kevin W. Hager, Western Washington University
Craig L. Moyer, Western Washington UniversityFollow
Kirsten Küsel, Friedrich Schiller Universitat, Jena
David Emerson, Bigelow Laboratory for Ocean Sciences

Document Type

Article

Publication Date

2017

Keywords

Zetaproteobacteria, Bacterial genomics, Microbiology

Abstract

Chemosynthetic Fe-oxidizing communities are common at diffuse-flow hydrothermal vents throughout the world’s oceans. The foundational members of these communities are the Zetaproteobacteria, a class of Proteobacteria that is primarily associated with ecosystems fueled by ferrous iron, Fe(II). We report here the discovery of two new isolates of Zetaproteobacteria isolated from the Mid-Atlantic Ridge (TAG-1), and the Mariana back-arc (SV-108), that are unique in that they can utilize either Fe(II) or molecular hydrogen (H₂) as sole electron donor and oxygen as terminal electron acceptor for growth. Both strains precipitated Fe-oxyhydroxides as amorphous particulates. The cell doubling time on H₂ vs Fe(II) for TAG-1 was 14.1 vs 21.8 h, and for SV-108 it was 16.3 vs 20 h, and it appeared both strains could use either H₂ or Fe(II) simultaneously. The strains were close relatives, based on genomic analysis, and both possessed genes for the uptake NiFe-hydrogenase required for growth on H_2. These two strains belong to Zetaproteobacteria operational taxonomic unit 9 (ZetaOTU9). A meta-analysis of public databases found ZetaOTU9 was only associated with Fe(II)-rich habitats, and not in other environments where known H₂-oxidizers exist. These results expand the metabolic repertoire of the Zetaproteobacteria, yet confirm that Fe(II) metabolism is the primary driver of their physiology and ecology

Publication Title

The ISME Journal

Volume

11

First Page

2624

Last Page

2636

DOI

10.1038/ismej.2017.132

Required Publisher's Statement

© The Author(s) 2017

Comments

Supplementary Information accompanies this paper on The ISME Journal website (http://www.nature.com/ismej)

Shareable link: https://rdcu.be/bieRm

Recommended Citation

Mori, Jiro F.; Scott, Jarrod J.; Hager, Kevin W.; Moyer, Craig L.; Küsel, Kirsten; and Emerson, David, "Physiological and ecological implications of an iron- or hydrogen-oxidizing member of the Zetaproteobacteria, Ghiorsea bivora, gen. nov., sp. nov." (2017). Biology Faculty and Staff Publications. 62.
https://cedar.wwu.edu/biology_facpubs/62

Subjects - Topical (LCSH)

Proteobacteria; Hydrogen bacteria; Hydrothermal vents--Microbiology; Bacterial diversity

Genre/Form

articles

Type

Text

Rights

Copying of this document in whole or in part is allowable only for scholarly purposes. It is understood, however, that any copying or publication of this document for commercial purposes, or for financial gain, shall not be allowed without the author’s written permission.

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-No Derivative Works 4.0 International License.

Language

English

Format

application/pdf