Presentation Title

Surface Plasmon Resonance Biosensor with a Biotin-Streptavidin Complex Based on Guided-Wave Plasmon Polariton Modes

Presentation Type

Poster

Abstract

Surface Plasmon Resonance (SPR) is the phenomenon in which charge density oscillations are excited at a metal-dielectric interface. SPR biosensors utilize surface plasmon polaritons (SPP), in which the charge oscillation is excited by an incident electromagnetic wave. The wavelength of light required to excite SPPs is highly dependent on the refractive index, and this change in excitation frequency can be detected through the use of Attenuated Total Reflectance (ATR). When a protein binding interaction takes place at the surface of the sample, the refractive index of the analyte is dramatically altered, leading to a shift in excitation wavelength as measured by ATR. We have recently developed a novel metal-insulator-metal structure capable of supporting Guided-Wave Plasmon Polariton Modes (GW-PPMs). In GW-PPMs, the central insulator is replaced with a higher refractive index than that of the substrate. In contrast to SPPs, GW-PPMs have the potential for increased propagation lengths. This structure shows promise in biosensor applications because it has been shown that higher propagation lengths lead to increased sensitivity for SPR biosensors. We will discuss both experimental and theoretical progress towards the production of a biosensor structure based on GW-PPMs that utilizes a biotin self-assembled monolayer (SAM) to bind the protein streptavidin onto the gold surface in order to modify the refractive index.

Start Date

6-5-2017 12:15 PM

End Date

6-5-2017 2:00 PM

Genre/Form

posters

Subjects - Topical (LCSH)

Surface plasmon resonance

Type

Event

Format

application/pdf

Language

English

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COinS
 
May 6th, 12:15 PM May 6th, 2:00 PM

Surface Plasmon Resonance Biosensor with a Biotin-Streptavidin Complex Based on Guided-Wave Plasmon Polariton Modes

Miller Hall

Surface Plasmon Resonance (SPR) is the phenomenon in which charge density oscillations are excited at a metal-dielectric interface. SPR biosensors utilize surface plasmon polaritons (SPP), in which the charge oscillation is excited by an incident electromagnetic wave. The wavelength of light required to excite SPPs is highly dependent on the refractive index, and this change in excitation frequency can be detected through the use of Attenuated Total Reflectance (ATR). When a protein binding interaction takes place at the surface of the sample, the refractive index of the analyte is dramatically altered, leading to a shift in excitation wavelength as measured by ATR. We have recently developed a novel metal-insulator-metal structure capable of supporting Guided-Wave Plasmon Polariton Modes (GW-PPMs). In GW-PPMs, the central insulator is replaced with a higher refractive index than that of the substrate. In contrast to SPPs, GW-PPMs have the potential for increased propagation lengths. This structure shows promise in biosensor applications because it has been shown that higher propagation lengths lead to increased sensitivity for SPR biosensors. We will discuss both experimental and theoretical progress towards the production of a biosensor structure based on GW-PPMs that utilizes a biotin self-assembled monolayer (SAM) to bind the protein streptavidin onto the gold surface in order to modify the refractive index.