Investigation of C3H Zinc Finger Gene Function Using CRISPR
Research Mentor(s)
Pillitteri, Lynn
Description
Zinc finger motifs are abundant and are known to have considerable variation in binding properties. In this study, we investigate the function of two C3H zinc finger proteins from Arabidopsis thaliana encoded by genes AT3G52440, AT5G06420 and AT1G01350. This family of proteins has been shown to be involved in protein-protein, protein-RNA, and protein-DNA interactions. Although many animal C3H zinc finger proteins have been extensively described, only a few family members have been characterized in plants. To better understand the function of these two zinc finger proteins, we employed CRISPR gene editing techniques to knock-out gene function. We used Agrobacterium mediated plant transformation to stably transfer CRISPR guide RNA and CAS9 into plants. The current progress of the phenotypic characterization of the generated CRISPR lines will be presented.
Document Type
Event
Start Date
17-5-2017 12:00 PM
End Date
17-5-2017 3:00 PM
Department
Biology
Genre/Form
student projects; posters
Subjects – Topical (LCSH)
Plant genetic engineering; Gene expression; Plant genetics
Type
Image
Rights
Copying of this document in whole or in part is allowable only for scholarly purposes. It is understood, however, that any copying or publication of this documentation for commercial purposes, or for financial gain, shall not be allowed without the author's written permission.
Language
English
Format
application/pdf
Investigation of C3H Zinc Finger Gene Function Using CRISPR
Zinc finger motifs are abundant and are known to have considerable variation in binding properties. In this study, we investigate the function of two C3H zinc finger proteins from Arabidopsis thaliana encoded by genes AT3G52440, AT5G06420 and AT1G01350. This family of proteins has been shown to be involved in protein-protein, protein-RNA, and protein-DNA interactions. Although many animal C3H zinc finger proteins have been extensively described, only a few family members have been characterized in plants. To better understand the function of these two zinc finger proteins, we employed CRISPR gene editing techniques to knock-out gene function. We used Agrobacterium mediated plant transformation to stably transfer CRISPR guide RNA and CAS9 into plants. The current progress of the phenotypic characterization of the generated CRISPR lines will be presented.