Investigating RNAi Biogenesis Pathway in Tetrahymena thermophila: RNA-Seq Analysis
Research Mentor(s)
Lee, Suzanne
Description
RNA interference is an essential post transcriptional regulatory mechanism for proper cellular function and organismal health. RNAi depends on small RNAs (sRNA) to direct this regulation. In T. thermophila there are multiple classes of sRNAs that depend on a variety of protein complexes for proper sRNA biogenesis. To further characterize the function and biogenesis of these sRNA classes, RNA-sequencing was performed on a variety of knockout strains in the sRNA biogenesis pathway. This study focuses on the results of gene ontologies and differential expression of genes in their the various knockout strains.
Document Type
Event
Start Date
May 2018
End Date
May 2018
Department
Biology
Genre/Form
student projects, posters
Subjects – Topical (LCSH)
RNA splicing; RNA--Metabolism; Bioinformatics
Type
Image
Rights
Copying of this document in whole or in part is allowable only for scholarly purposes. It is understood, however, that any copying or publication of this document for commercial purposes, or for financial gain, shall not be allowed without the author’s written permission.
Language
English
Format
application/pdf
Investigating RNAi Biogenesis Pathway in Tetrahymena thermophila: RNA-Seq Analysis
RNA interference is an essential post transcriptional regulatory mechanism for proper cellular function and organismal health. RNAi depends on small RNAs (sRNA) to direct this regulation. In T. thermophila there are multiple classes of sRNAs that depend on a variety of protein complexes for proper sRNA biogenesis. To further characterize the function and biogenesis of these sRNA classes, RNA-sequencing was performed on a variety of knockout strains in the sRNA biogenesis pathway. This study focuses on the results of gene ontologies and differential expression of genes in their the various knockout strains.