Event Title

Investigating RNAi Biogenesis Pathway in Tetrahymena thermophila: RNA-Seq Analysis

Research Mentor(s)

Suzanne Lee

Description

RNA interference is an essential post transcriptional regulatory mechanism for proper cellular function and organismal health. RNAi depends on small RNAs (sRNA) to direct this regulation. In T. thermophila there are multiple classes of sRNAs that depend on a variety of protein complexes for proper sRNA biogenesis. To further characterize the function and biogenesis of these sRNA classes, RNA-sequencing was performed on a variety of knockout strains in the sRNA biogenesis pathway. This study focuses on the results of gene ontologies and differential expression of genes in their the various knockout strains.

Document Type

Event

Start Date

May 2018

End Date

May 2018

Location

Biology

Rights

Copying of this document in whole or in part is allowable only for scholarly purposes. It is understood, however, that any copying or publication of this document for commercial purposes, or for financial gain, shall not be allowed without the author’s written permission.

Language

English

Format

application/pdf

This document is currently not available here.

Share

COinS
 
May 17th, 9:00 AM May 17th, 12:00 PM

Investigating RNAi Biogenesis Pathway in Tetrahymena thermophila: RNA-Seq Analysis

Biology

RNA interference is an essential post transcriptional regulatory mechanism for proper cellular function and organismal health. RNAi depends on small RNAs (sRNA) to direct this regulation. In T. thermophila there are multiple classes of sRNAs that depend on a variety of protein complexes for proper sRNA biogenesis. To further characterize the function and biogenesis of these sRNA classes, RNA-sequencing was performed on a variety of knockout strains in the sRNA biogenesis pathway. This study focuses on the results of gene ontologies and differential expression of genes in their the various knockout strains.