Sea star microbiome and potential role in sea star wasting disease
Research Mentor(s)
Brodhagen, Marion (Marion L.)
Description
During the summer of 2013, Pisaster ochraceus experienced a mass die-off event due to a poorly understood syndrome called sea star wasting disease (SSWD). Symptoms prior to death included lesions, limb detachment, and tissue degradation. Since then, SSWD has spread to much of the North American Pacific Coast and has been found in over twenty species of sea stars. The focus of our research was to explore the role of sea star associated bacteria in SSWD. We collected several hundred bacterial isolates from the epidermal surfaces of healthy and diseased sea stars that can degrade collagen, the major component of sea star epidermis. We assigned tentative identifications via 16S rRNA gene sequencing and comparison with extant databases. We then explored the community interactions among these isolates with an antagonism assay involving co-culture in Petri plates. Finally, we collected endosymbionts from beneath the epidermal surface by growing out bacteria from surface-sterilized tissues, and assigned tentative identification as above. While our research does not identify a causal agent of SSWD, it provides a foundation for understanding the microbiome of sea star epidermal tissues. We hypothesize that bacteria within the sea star microbiome include both protective and opportunistically pathogenic species.
Document Type
Event
Start Date
17-5-2018 12:00 AM
End Date
17-5-2018 12:00 AM
Department
Biology
Genre/Form
student projects, posters
Subjects – Topical (LCSH)
Pisaster ochraceus--Diseases and pests--Pacific Coast (America); Chronic wasting disease--Pacific Coast (America); Pisaster ochraceus--Pathogens--Pacific Coast (America)
Geographic Coverage
Pacific Coast (America)
Type
Image
Rights
Copying of this document in whole or in part is allowable only for scholarly purposes. It is understood, however, that any copying or publication of this document for commercial purposes, or for financial gain, shall not be allowed without the author’s written permission.
Language
English
Format
application/pdf
Sea star microbiome and potential role in sea star wasting disease
During the summer of 2013, Pisaster ochraceus experienced a mass die-off event due to a poorly understood syndrome called sea star wasting disease (SSWD). Symptoms prior to death included lesions, limb detachment, and tissue degradation. Since then, SSWD has spread to much of the North American Pacific Coast and has been found in over twenty species of sea stars. The focus of our research was to explore the role of sea star associated bacteria in SSWD. We collected several hundred bacterial isolates from the epidermal surfaces of healthy and diseased sea stars that can degrade collagen, the major component of sea star epidermis. We assigned tentative identifications via 16S rRNA gene sequencing and comparison with extant databases. We then explored the community interactions among these isolates with an antagonism assay involving co-culture in Petri plates. Finally, we collected endosymbionts from beneath the epidermal surface by growing out bacteria from surface-sterilized tissues, and assigned tentative identification as above. While our research does not identify a causal agent of SSWD, it provides a foundation for understanding the microbiome of sea star epidermal tissues. We hypothesize that bacteria within the sea star microbiome include both protective and opportunistically pathogenic species.