Controlling the Equilibrium of Sortase-Mediated Ligations to Modify Proteins and Build Peptide Oligomers
Research Mentor(s)
Antos, John M.
Description
The sortase A enzyme is a powerful protein engineering tool which allows for site-specific ligations of peptide and protein building blocks containing an LPXTG motif. However, ligation reversibility lowers the efficiency of this approach, and limits the types of structures that can be generated using sortase-based methods. Here we describe efforts to control the equilibrium of sortase-mediated ligations by sequestering certain reaction by-products or by tuning the solubility of ligation products. In this way, we are able drive ligation reactions to high levels of completion without the need for excess reagents. These strategies have proven effective in boosting the conversion of protein modification reactions, as well as promoting the formation of unique peptide oligomers that have the potential to serve as new biomaterials scaffolds.
Document Type
Event
Start Date
15-5-2019 9:00 AM
End Date
15-5-2019 5:00 PM
Location
Carver Gym (Bellingham, Wash.)
Department
Chemistry
Genre/Form
student projects, posters
Subjects – Topical (LCSH)
Membrane proteins; Peptides--Synthesis; Ligases; Oligomers
Type
Image
Rights
Copying of this document in whole or in part is allowable only for scholarly purposes. It is understood, however, that any copying or publication of this document for commercial purposes, or for financial gain, shall not be allowed without the author’s written permission.
Language
English
Format
application/pdf
Controlling the Equilibrium of Sortase-Mediated Ligations to Modify Proteins and Build Peptide Oligomers
Carver Gym (Bellingham, Wash.)
The sortase A enzyme is a powerful protein engineering tool which allows for site-specific ligations of peptide and protein building blocks containing an LPXTG motif. However, ligation reversibility lowers the efficiency of this approach, and limits the types of structures that can be generated using sortase-based methods. Here we describe efforts to control the equilibrium of sortase-mediated ligations by sequestering certain reaction by-products or by tuning the solubility of ligation products. In this way, we are able drive ligation reactions to high levels of completion without the need for excess reagents. These strategies have proven effective in boosting the conversion of protein modification reactions, as well as promoting the formation of unique peptide oligomers that have the potential to serve as new biomaterials scaffolds.