Actin Filament Binding in Plant Protein Villin-4
Research Mentor(s)
Dr. Sergey Smirnov
Description
Proteins, such as villin regulate the shape and function of biological cells in most higher organisms including vertebrates and plants. Biochemically, villin proteins bind actin filaments which regulate structural pillars of the cellular cytoskeleton. Generally, plant villins are not as well studied as vertebrate villins. Recently a novel structure within plant villins was discovered in the form of a large internal disordered region referred to as VLN4 Linker. In this experiment we used actin spin-down assays, SDS-PAGE electrophoresis and HPLC to probe the capacity of the VLN4 Linker to bind actin filaments.
Document Type
Event
Start Date
May 2022
End Date
May 2022
Location
Carver Gym (Bellingham, Wash.)
Department
CSE - Chemistry
Genre/Form
student projects; posters
Type
Image
Rights
Copying of this document in whole or in part is allowable only for scholarly purposes. It is understood, however, that any copying or publication of this document for commercial purposes, or for financial gain, shall not be allowed without the author’s written permission.
Language
English
Format
application/pdf
Actin Filament Binding in Plant Protein Villin-4
Carver Gym (Bellingham, Wash.)
Proteins, such as villin regulate the shape and function of biological cells in most higher organisms including vertebrates and plants. Biochemically, villin proteins bind actin filaments which regulate structural pillars of the cellular cytoskeleton. Generally, plant villins are not as well studied as vertebrate villins. Recently a novel structure within plant villins was discovered in the form of a large internal disordered region referred to as VLN4 Linker. In this experiment we used actin spin-down assays, SDS-PAGE electrophoresis and HPLC to probe the capacity of the VLN4 Linker to bind actin filaments.