A systems approach of diatom responses to ocean acidification
Presentation Abstract
Diatoms are unicellular photosynthetic eukaryotic algae that account for 40% of the marine primary production, they play a critical role in the marine carbon cycle, and over geological times have influenced the global climate. Our objective is to understand what the most important effects of climate change and ocean acidification will be on diatoms using a systems approach. The goal of a systems approach is to integrate all the measurements in order to formulate models that recapitulate all the observations and to predict new behavior in response to new environmental perturbations. We have conducted a genome-wide transcription profiling of the model diatom Thalassiosira pseudonana during growth at two CO2 levels: present day (400 ppm) and a doubling of the CO2 level (800 ppm), reflecting the projected scenario for the 21st century. In this presentation we will focus on replicate analyses of the physiological and molecular modes associated with the cells state (exponential and nutrient replete, stationary and nutrient depleted) under the two CO2 conditions. Our results show that most of the expressed genes associated with the difference in CO2 growth are unknown, not annotated or hypothetical genes (800 genes). These genes changed in expression mostly under stationary phase, when nutrients were depleted from the media and the cells were undergoing starvation.
Session Title
Session S-05A: Frontiers of Ocean Acidification Research in the Salish Sea
Conference Track
Ocean Acidification
Conference Name
Salish Sea Ecosystem Conference (2014 : Seattle, Wash.)
Document Type
Event
Start Date
1-5-2014 10:30 AM
End Date
1-5-2014 12:00 PM
Location
Room 615-616-617
Genre/Form
conference proceedings; presentations (communicative events)
Contributing Repository
Digital content made available by University Archives, Heritage Resources, Western Libraries, Western Washington University.
Subjects – Topical (LCSH)
Thalassiosira--Effect of water acidification on--Salish Sea (B.C. and Wash.); Thalassiosira--Climatic factors--Salish Sea (B.C. and Wash.)
Geographic Coverage
Salish Sea (B.C. and Wash.)
Rights
This resource is displayed for educational purposes only and may be subject to U.S. and international copyright laws. For more information about rights or obtaining copies of this resource, please contact University Archives, Heritage Resources, Western Libraries, Western Washington University, Bellingham, WA 98225-9103, USA (360-650-7534; heritage.resources@wwu.edu) and refer to the collection name and identifier. Any materials cited must be attributed to the Salish Sea Ecosystem Conference Records, University Archives, Heritage Resources, Western Libraries, Western Washington University.
Type
Text
Language
English
Format
application/pdf
A systems approach of diatom responses to ocean acidification
Room 615-616-617
Diatoms are unicellular photosynthetic eukaryotic algae that account for 40% of the marine primary production, they play a critical role in the marine carbon cycle, and over geological times have influenced the global climate. Our objective is to understand what the most important effects of climate change and ocean acidification will be on diatoms using a systems approach. The goal of a systems approach is to integrate all the measurements in order to formulate models that recapitulate all the observations and to predict new behavior in response to new environmental perturbations. We have conducted a genome-wide transcription profiling of the model diatom Thalassiosira pseudonana during growth at two CO2 levels: present day (400 ppm) and a doubling of the CO2 level (800 ppm), reflecting the projected scenario for the 21st century. In this presentation we will focus on replicate analyses of the physiological and molecular modes associated with the cells state (exponential and nutrient replete, stationary and nutrient depleted) under the two CO2 conditions. Our results show that most of the expressed genes associated with the difference in CO2 growth are unknown, not annotated or hypothetical genes (800 genes). These genes changed in expression mostly under stationary phase, when nutrients were depleted from the media and the cells were undergoing starvation.
