Using Real-Time Polymerase Chain Reaction to Determine Spatial Distribution of Multiple Species of Shellfish
Presentation Abstract
The success of both wild and cultured shellfish populations is dependent upon recruitment of planktonic larvae. Due to issues of cost, time, expertise and inaccuracy associated with bivalve identification using microscopy, real-time polymerase chain reaction is being employed to identify and quantify larvae using DNA technology. We are quantifying species-specific abundance and distribution of four commercially important species using novel approaches. Environmental samples were collected via two rounds of in-situ pumping at four locations in intertidal waters in Washington State. Pumping was performed at two depths: near the water surface and above the sea floor and at two times: before sunrise and sunset, in order to determine the spatial and temporal distribution of bivalve larvae. Genetic assays for Pacific geoduck clam (Panopea generosa) Olympia oyster (Ostrea lurida), Pacific oyster (Crassostrea gigas) and Manila clam (Venerupis philippinarum) have been designed. The collected field samples are currently undergoing qPCR quantification using these assays. Results will be analyzed to determine cross-species patterns or species-specific behavior in larval distribution throughout Washington State. This information will provide a comprehensive snapshot of the larvae of multiple shellfish species in Washington. Additionally, this information may further be utilized by hatcheries by providing the best times and locations for hatcheries to plant cultured seeds and substrate and by researchers studying the effects of localized ocean acidification.
Session Title
General protection, remediation and restoration topics
Keywords
Keywords: quantitative polymerase chain reaction, Panopea generosa, Crassostrea gigas, Ruditapes philippinarum, Ostrea lurida
Conference Track
Protection, Remediation, & Restoration
Conference Name
Salish Sea Ecosystem Conference (2016 : Vancouver, B.C.)
Document Type
Event
Start Date
2016 12:00 AM
Location
2016SSEC
Type of Presentation
Poster
Genre/Form
presentations (communicative events)
Contributing Repository
Digital content made available by University Archives, Heritage Resources, Western Libraries, Western Washington University.
Subjects – Topical (LCSH)
Shellfish--Larvae--Washington (State)--Geographical distribution
Geographic Coverage
Salish Sea (B.C. and Wash.)
Rights
This resource is displayed for educational purposes only and may be subject to U.S. and international copyright laws. For more information about rights or obtaining copies of this resource, please contact University Archives, Heritage Resources, Western Libraries, Western Washington University, Bellingham, WA 98225-9103, USA (360-650-7534; heritage.resources@wwu.edu) and refer to the collection name and identifier. Any materials cited must be attributed to the Salish Sea Ecosystem Conference Records, University Archives, Heritage Resources, Western Libraries, Western Washington University.
Type
Text
Language
English
Format
application/pdf
Using Real-Time Polymerase Chain Reaction to Determine Spatial Distribution of Multiple Species of Shellfish
2016SSEC
The success of both wild and cultured shellfish populations is dependent upon recruitment of planktonic larvae. Due to issues of cost, time, expertise and inaccuracy associated with bivalve identification using microscopy, real-time polymerase chain reaction is being employed to identify and quantify larvae using DNA technology. We are quantifying species-specific abundance and distribution of four commercially important species using novel approaches. Environmental samples were collected via two rounds of in-situ pumping at four locations in intertidal waters in Washington State. Pumping was performed at two depths: near the water surface and above the sea floor and at two times: before sunrise and sunset, in order to determine the spatial and temporal distribution of bivalve larvae. Genetic assays for Pacific geoduck clam (Panopea generosa) Olympia oyster (Ostrea lurida), Pacific oyster (Crassostrea gigas) and Manila clam (Venerupis philippinarum) have been designed. The collected field samples are currently undergoing qPCR quantification using these assays. Results will be analyzed to determine cross-species patterns or species-specific behavior in larval distribution throughout Washington State. This information will provide a comprehensive snapshot of the larvae of multiple shellfish species in Washington. Additionally, this information may further be utilized by hatcheries by providing the best times and locations for hatcheries to plant cultured seeds and substrate and by researchers studying the effects of localized ocean acidification.
Comments
Keywords: quantitative polymerase chain reaction, Panopea generosa, Crassostrea gigas, Ruditapes philippinarum, Ostrea lurida