Presentation Abstract
Harmful algal toxins have been well recognized as public health threats (James et al., 2010; Van Dolah, 2000), and a multitude of measures to prevent harmful algal blooms (HABs) derived health risks have been proposed and implemented (Trainer and Hardy, 2015). Coastal communities such as the Skokomish Indian Tribe, consuming a large amount of shellfish to meet their dietary needs are particularly vulnerable to such risks. Washington Department of Health (DOH) has been monitoring marine algal toxins in Puget Sound including coastal areas by collecting shellfish samples followed by mouse-based toxin analysis. To address on-going and future marine algal toxin issues in Hood Canal in the face of changing climate, the Skokomish Indian Tribe launched a research project to develop chemical analysis protocols for representative toxins using LC/MS and monitor algal bloom events. Eight toxins were selected for method development and monitoring Protocols for solid phase extraction combined with LC/MS analysis were developed. Multiple reaction monitoring (MRM) protocols for individual toxin compounds were developed and used to quantify toxin concentrations against standard curves established using certified toxin standard materials. From June through September 2017, we conducted weekly monitoring of algal toxin concentrations in sea water and phytoplankton samples from 13 monitoring sites, including Sequim Bay. No okadaic acid and dinophysistoxin-2 were detected throughout the sampling period, while a few alga toxin compounds were fluctuating over time. The details of spatial and temporal distributions of selected algal toxins in Hood Canal obtained from Summer of 2017 will be presented with a discussion about future directions for this initiative. The newly developed algal toxin analysis using LC/MS offers a promising tool to address some of public health and environmental issues associated with marine algal toxins in Hood Canal as well as possibly in the Salish Sea.
Session Title
Harmful Phytoplankton in the Salish Sea: Part II
Keywords
LC/MS, Marine algal toxins
Conference Track
SSE5: Climate Change: Impacts, Adaptation, and Research
Conference Name
Salish Sea Ecosystem Conference (2018 : Seattle, Wash.)
Document Type
Event
SSEC Identifier
SSE5-108
Start Date
4-4-2018 4:30 PM
End Date
4-4-2018 4:45 PM
Type of Presentation
Oral
Genre/Form
presentations (communicative events)
Contributing Repository
Digital content made available by University Archives, Heritage Resources, Western Libraries, Western Washington University.
Subjects – Topical (LCSH)
Algal blooms--Washington (State)--Hood Canal--Analysis; Algal blooms--Monitoring--Washington (State)--Hood Canal
Subjects – Names (LCNAF)
Washington (State). Department of Health
Geographic Coverage
Hood Canal (Wash.); Salish Sea (B.C. and Wash.)
Rights
This resource is displayed for educational purposes only and may be subject to U.S. and international copyright laws. For more information about rights or obtaining copies of this resource, please contact University Archives, Heritage Resources, Western Libraries, Western Washington University, Bellingham, WA 98225-9103, USA (360-650-7534; heritage.resources@wwu.edu) and refer to the collection name and identifier. Any materials cited must be attributed to the Salish Sea Ecosystem Conference Records, University Archives, Heritage Resources, Western Libraries, Western Washington University.
Type
Text
Language
English
Format
application/pdf
Included in
Fresh Water Studies Commons, Marine Biology Commons, Natural Resources and Conservation Commons, Terrestrial and Aquatic Ecology Commons
Development and application of LC/MS based analysis for marine algal toxins in Hood Canal
Harmful algal toxins have been well recognized as public health threats (James et al., 2010; Van Dolah, 2000), and a multitude of measures to prevent harmful algal blooms (HABs) derived health risks have been proposed and implemented (Trainer and Hardy, 2015). Coastal communities such as the Skokomish Indian Tribe, consuming a large amount of shellfish to meet their dietary needs are particularly vulnerable to such risks. Washington Department of Health (DOH) has been monitoring marine algal toxins in Puget Sound including coastal areas by collecting shellfish samples followed by mouse-based toxin analysis. To address on-going and future marine algal toxin issues in Hood Canal in the face of changing climate, the Skokomish Indian Tribe launched a research project to develop chemical analysis protocols for representative toxins using LC/MS and monitor algal bloom events. Eight toxins were selected for method development and monitoring Protocols for solid phase extraction combined with LC/MS analysis were developed. Multiple reaction monitoring (MRM) protocols for individual toxin compounds were developed and used to quantify toxin concentrations against standard curves established using certified toxin standard materials. From June through September 2017, we conducted weekly monitoring of algal toxin concentrations in sea water and phytoplankton samples from 13 monitoring sites, including Sequim Bay. No okadaic acid and dinophysistoxin-2 were detected throughout the sampling period, while a few alga toxin compounds were fluctuating over time. The details of spatial and temporal distributions of selected algal toxins in Hood Canal obtained from Summer of 2017 will be presented with a discussion about future directions for this initiative. The newly developed algal toxin analysis using LC/MS offers a promising tool to address some of public health and environmental issues associated with marine algal toxins in Hood Canal as well as possibly in the Salish Sea.
