Senior Project Advisor

Prody, Gerry

Document Type

Project

Publication Date

Spring 2000

Keywords

Satellite RNA

Abstract

The satellite RNA found with tobacco ringspot virus is capable of attenuating the symptoms of viral infection. Although the satellite appears within viral capsids after several passages of infected plants, the origin of this satellite RNA sequence has not been previously determined. Earlier work indicated a potential mitochondrial origin of this sequence. Using satellite specific primers and the polymerase chain reaction (PCR), the mitochondrial DNA of uninfected tobacco cells was probed for sequence corresponding to the satellite RNA. A new protocol was adapted for the isolation of mitochondrial DNA in which specific isolation of protoplasts and mitochondria was not required. Although two PCR products were isolated, sequencing of these products and analysis with the MacVector 3.5 Sequence Analysis program showed no homology with the budblight strain of tobacco ringspot virus satellite RNA.

Department

Chemistry

Subjects - Topical (LCSH)

Tobacco ringspot virus; RNA viruses--Reproduction; Mitochondrial DNA

Genre/Form

student projects; term papers

Type

Text

Rights

Copying of this document in whole or in part is allowable only for scholarly purposes. It is understood, however, that any copying or publication of this document for commercial purposes, or for financial gain, shall not be allowed without the author’s written permission.

Rights Statement

http://rightsstatements.org/vocab/InC/1.0/

Language

English

Format

application/pdf

Download

Included in

Chemistry Commons

Share

COinS