Authors

Rose Border

Senior Project Advisor

Nick Galati

Document Type

Project - Campus-only Access

Publication Date

Spring 2024

Keywords

Golgi, centrosome, centriolar satellites, cell biology, fluorescence, microscopy, cell culture, live imaging

Abstract

The Golgi Apparatus is a complex system of vesicle layers serving various functions in the cell. The centrosome is a major microtubule-organizing center that serves a vital role in mitosis and as the base of the cilium, a vital signaling organelle. The Golgi and centrosome appear to impact the localization of one another, but the mechanisms that govern their reciprocal localization are poorly understood. The Galati Lab is interested in how centriolar satellites, proteinaceous granules which serve a vital role in ciliogenesis and proper ciliary function (Odabaşı et. al. 2019), are organized. The Galati lab has pursued numerous staining experiments, but now seeks greater understanding of the in vivo dynamics of the Golgi-centrosome-satellite relationship. This assay presents a foundation for more experiments to be carried out in live cells.

Department

Biology

Type

Text

Rights

Copying of this document in whole or in part is allowable only for scholarly purposes. It is understood, however, that any copying or publication of this document for commercial purposes, or for financial gain, shall not be allowed without the author’s written permission.

Language

English

Format

application/pdf

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