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Date of Award

Fall 2024

Document Type

Masters Thesis

Degree Name

Master of Science (MS)

Department

Chemistry

First Advisor

Lemkau, Karin

Second Advisor

Montaño, Manuel D.

Third Advisor

O'Neil, Gregory (Gregory W.)

Abstract

Residues from oil spills are deposited in coastal environments and can persist for decades in the environment. Following an oil spill, analysis of homogenized oil residues scraped off rocks and debris from coastlines is common practice. This analysis of bulk oil samples may not accurately reflect the environmental availability of the oil components present in the residue. Weathering processes such as evaporation, dissolution, photodegradation, and biodegradation can lead to the formation of a visually distinct skin on the oil surface, an observation we term differential weathering of an oil residue. This distinct surface layer may lead to physical shielding of the bulk oil below resulting in differences in the chemical composition between the surface and bottom layers of a residue, which may have toxicological implications. Here we assess the implications of traditional bulk analysis methods by sampling oil in vertically sliced sections to examine the chemical differences between surface and deeper layers of a simulated heavy fuel oil residue. We used gas chromatography with flame ionization detection and mass spectrometry to quantify polycyclic aromatic hydrocarbon (PAH) and total petroleum hydrocarbon (TPH) concentrations in each layer. Surface layers exhibited more extensive weathering compared to bottom layers in a more viscous oil residue. Lower viscosity oil residues did not exhibit this differential weathering. Fourier transform infrared spectroscopy and elemental analysis was used to explore oxidation with depth in oil samples. A toxicity assay using water accommodated fractions from oil residues was used to examine changes in the lethality of weathered oil with depth. Oxidation was observed in all irradiated oil samples with greater oxidation observed in surface layers. Oxidation did not translate into differences in toxicity to the marine algae Isochrysis galbana. Future work will explore differential weathering over a broader range of oil viscosities and include expanded toxicity assays at more environmentally relevant concentrations.

Type

Text

Keywords

analytical chemistry, environmental chemistry, crude oil, heavy fuel oil, HFO, oil spill, photodegradation, polycyclic aromatic hydrocarbons, PAHs, total petroleum hydrocarbons, TPHs, toxicity

Publisher

Western Washington University

OCLC Number

1474572035

Subject – LCSH

Petroleum--Toxicology; Hydrocarbons--Analysis; Polycyclic aromatic hydrocarbons--Analysis; Petroleum--Testing; Oil spills--Environmental aspects

Format

application/pdf

Genre/Form

masters theses

Language

English

Rights

Copying of this document in whole or in part is allowable only for scholarly purposes. It is understood, however, that any copying or publication of this document for commercial purposes, or for financial gain, shall not be allowed without the author’s written permission.

Rights Statement

http://rightsstatements.org/vocab/UND/1.0/

Available for download on Tuesday, December 02, 2025

Included in

Chemistry Commons

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