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Date Permissions Signed
5-22-2012
Date of Award
2012
Document Type
Masters Thesis
Degree Name
Master of Science (MS)
Department
Biology
First Advisor
Pillitteri, Lynn
Second Advisor
Young, Jeff C. (Jeffery C.)
Third Advisor
Leaf, David Scott, 1955-
Abstract
Stomata are found on the surfaces of land plants and are crucial for regulating gas exchange between plants and the atmosphere. These structures are composed of a pore that is surrounded by two specialized guard cells. The critical importance of stomata in providing CO2 uptake while controlling the release of water has made them a prime target for improvement of plant productivity and water use efficiency. In Arabidopsis, the production of a mature stomata requires the expression of the basic helix-loop-helix (bHLH) master regulatory gene, MUTE. The function of MUTE and its expression pattern have been characterized. In this study, promoter deletion analysis of MUTE was performed to identify specific regions that control the spatiotemporal expression of MUTE. Expression vectors with truncated promoter segments driving the expression of GFP (Green Fluorescent Protein) were transformed into Arabidopsis. A region of 110-bp was identified as required for MUTE expression. This 110-bp region was further analyzed for putative cis-elements using publicly available databases. Seven possible CREs (CIS-Regulatory Elements) were identified which are known binding sites for transcription factors involved in development, dehydration, light regulation, and stomatal pore physiology. Three of these putative elements were chosen for targeted mutagenesis to produce GFP expression vectors for future molecular characterization in A.thaliana. This work provides novel insight into the regulatory mechanism driving MUTE expression and offers new tools for identifying key regulatory elements in the future.
Type
Text
DOI
https://doi.org/10.25710/rsff-jq61
Publisher
Western Washington University
OCLC Number
795241712
Subject – LCSH
Arabidopsis--Genetics; Stomata; Promoters (Genetics); Mutagenesis; Gene expression
Format
application/pdf
Genre/Form
masters theses
Language
English
Rights
Copying of this document in whole or in part is allowable only for scholarly purposes. It is understood, however, that any copying or publication of this thesis for commercial purposes, or for financial gain, shall not be allowed without the author's written permission.
Recommended Citation
Mahoney, Aaron, "Promoter deletion analysis and identification of putative CIS-elements of MUTE in Arabidopsis" (2012). WWU Graduate School Collection. 212.
https://cedar.wwu.edu/wwuet/212