Authors

Emma Jerome

Senior Project Advisor

Jeff Cantle

Document Type

Project

Publication Date

Winter 2023

Keywords

CRISPR, Huntington's disease, gene editing

Abstract

This flowchart outlines the experimental methodology used to create and validate a gene knockout in an immortalized cell line. CRISPR/Cas9 gene editing technology was employed to inactivate the gene that encodes a protein of interest previously found to interact with huntingtin, the protein implicated in Huntington’s disease. The resulting cell line can be used for future protein-protein interaction research to better understand the function of huntingtin. This work is crucial for understanding the loss-of-function phenotypes thought to contribute to Huntington’s disease pathology. The identity of the protein of interest is proprietary information and is withheld from this outline.

Department

Psychology

Subjects - Topical (LCSH)

CRISPR-associated protein 9; Huntington's disease--Research; Gene editing

Type

Text

Rights

Copying of this document in whole or in part is allowable only for scholarly purposes. It is understood, however, that any copying or publication of this document for commercial purposes, or for financial gain, shall not be allowed without the author’s written permission.

Language

English

Format

application/pdf

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