Senior Project Advisor
Jeff Cantle
Document Type
Project
Publication Date
Winter 2023
Keywords
CRISPR, Huntington's disease, gene editing
Abstract
This flowchart outlines the experimental methodology used to create and validate a gene knockout in an immortalized cell line. CRISPR/Cas9 gene editing technology was employed to inactivate the gene that encodes a protein of interest previously found to interact with huntingtin, the protein implicated in Huntington’s disease. The resulting cell line can be used for future protein-protein interaction research to better understand the function of huntingtin. This work is crucial for understanding the loss-of-function phenotypes thought to contribute to Huntington’s disease pathology. The identity of the protein of interest is proprietary information and is withheld from this outline.
Department
Psychology
Recommended Citation
Jerome, Emma, "Creating a Knockout Cell Line: Using CRISPR/Cas9 as a Tool for Huntington's Disease Research" (2023). WWU Honors College Senior Projects. 640.
https://cedar.wwu.edu/wwu_honors/640
Subjects - Topical (LCSH)
CRISPR-associated protein 9; Huntington's disease--Research; Gene editing
Type
Text
Rights
Copying of this document in whole or in part is allowable only for scholarly purposes. It is understood, however, that any copying or publication of this document for commercial purposes, or for financial gain, shall not be allowed without the author’s written permission.
Language
English
Format
application/pdf