Senior Project Advisor
CRISPR, Huntington's disease, gene editing
This flowchart outlines the experimental methodology used to create and validate a gene knockout in an immortalized cell line. CRISPR/Cas9 gene editing technology was employed to inactivate the gene that encodes a protein of interest previously found to interact with huntingtin, the protein implicated in Huntington’s disease. The resulting cell line can be used for future protein-protein interaction research to better understand the function of huntingtin. This work is crucial for understanding the loss-of-function phenotypes thought to contribute to Huntington’s disease pathology. The identity of the protein of interest is proprietary information and is withheld from this outline.
Jerome, Emma, "Creating a Knockout Cell Line: Using CRISPR/Cas9 as a Tool for Huntington's Disease Research" (2023). WWU Honors College Senior Projects. 640.
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